PDE4B antibody (AA 500-736)

Catalog No. ABIN1869725

Product Details anti-PDE4B Antibody

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Binding Specificity: AA 500-736

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Phosphodiesterase 4B, cAMP Specific (PDE4B)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDE4B. It has been selected for its ability to recognize PDE4B in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phosphodiesterase 4B, cAMP Specific (PDE4B) corresdonding to Lys500~Thr736 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: Phosphodiesterase 4B, cAMP Specific (PDE4B Products)

Synonyms: dpde4 antibody, pde4b5 antibody, pdeivb antibody, Dpde4 antibody, R74983 antibody, dunce antibody, PDE4/IVb antibody, Pde4B1 antibody, Pde4B2 antibody, Pde4B3 antibody, Pde4B4 antibody, DPDE4 antibody, PDE4B5 antibody, PDEIVB antibody, phosphodiesterase 4B antibody, phosphodiesterase 4B, cAMP specific antibody, phosphodiesterase 4B S homeolog antibody, pde4b antibody, PDE4B antibody, Pde4b antibody, pde4b.S antibody

Background: DPDE4, PDE32, Dunce(Drosophila)Homolog Phosphodiesterase E4, cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction