MRPr1 reacts with an epitope of Multidrug Resistance Protein 1 (MRP1, ABCC1), a 180-195 kDa transmembrane transporter protein overexpressed in various human non-P-glycoprotein MDR tumor cell lines. MRPr1 does not cross-react with human MDR1 or MDR3 gene products. Species Reactivity: Human. Others not tested.
0.22 μm filtered
Bacterial fusion protein of MRP containing a segment of 168 amino acids located in the amino-proximal half of the protein.Mouse myeloma (SP2/0) fused with immunized rat (outbred Wistar strain) lymph node cells.
ELISA, FACS, WB
Flow cytometry: After fixing cells in 10% (v/v) Lysing solution, use MRPr1 at a 1:20-1:50 dilution followed by FITC-conjugated anti-rat IgG secondary antibody. Immunocytochemistry: For acetone-fixed cell preparation use at a 1:20-1:50 dilution. Immunohistochemistry: For acetone-fixed frozen sections, use at a 1:20-1:50 dilution. For formalin-fixed, paraffin-embedded tissues, use at a 1:20 dilution. For paraffin-embedded sections, a 0.01 M citrate pretreatment for 10 min. at 100 °C may increase the performance of MRPr1. Western blot: Use at a 1:20-1:50 dilution, followed by anti-rat-HRP. The optimal dilution for a specific application should be determined by the researcher.
For Research Use only
1 mL (>200 tests) or 0.5 mL (>100 tests) monoclonal antibody at ~250 µg IgG/mL in serum-free tissue culture supernatant with protein stabilizer and 0.1% sodium azide. Filtered through a 0.22 µm filter. Culture medium: RPMI-1640, supplemented with Nutrid
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.