QCRL-4 reacts with a conformation-dependent internal epitope of MRP1, a 190 kDa trans-membrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines. QCRL-4 inhibits the ATP-dependent transport activity of MRP1 in inside-out membrane vesicles and its epitope has been localized to the second nucleotide binding domain of MRP1 between amino acids 1294 and 1531. QCRL-4 does not cross-react with human MDR1 and MDR3 gene products. Species Reactivity: Human. May crossreact with monkey, mouse and rat. Others not tested.
0.22 μm filtered
Non-denatured membranes prepared from the human small cell lung cancer cell line H69AR, which highly overexpresses MRP1.The hybridoma cell line was obtained by fusion of SP2/O mouse myeloma cells with spleen cells from an immunized mouse.
Flow Cytometry: Use at a dilution of 1:20 - 1:40 after fixing cells in 0.5% paraformaldehyde, followed by fluorochrome conjugated anti-mouse reagent. Immunocytochemistry: Use on paraformaldehyde (0.5% in PBS) fixed cytospin preparations. Immunoprecipitation Western Blot: NOT REACTIVE Dot Blot: Non-denatured only, use at 1:10,000 dilution. Functional Activity: Transport inhibition. The optimal dilution for a specific application should be determined by the researcher.
For Research Use only
1 mL containing purified antibody (40 µg/mL IgG1) with 0.7% BSA and 0.1% NaN3 in PBS, 0.22 µM filtered.
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.