In immunoblots most human keratins are recognized. Cross-reactivity not detectable with the following: neurofilament, vimentin, GFAP, desmin. Species Reactivity: Human, mouse and rat. Others not tested.
Immunofluorescence Immunohistochemistry: For frozen sections use at approximately 1:50. For paraffin-embedded tissues use at approximately 1:10. Western blot: Positive control for Western blot: human callus or keratinocytes. The optimal dilution for a specific application should be determined by the researcher.
For Research Use only
1 ml culture supernatant with approximately 25 µg antibody/ml and 10 mM sodium azide with 1% FCS.
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.