ATP5F1 antibody (AA 161-195)

Catalog No. ABIN1881090

Product Details anti-ATP5F1 Antibody

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Binding Specificity: AA 161-195

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATP5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-195 amino acids from the Central region of human ATP5F1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ATP5F1 antibody (ABIN1881090)

Burkard, Planyavsky, Kaupe, Breitwieser, Bürckstümmer, Bennett, Superti-Furga, Colinge: "Initial characterization of the human central proteome." in: BMC systems biology, Vol. 5, pp. 17, (2011) (PubMed).

Choudhary, Kumar, Gnad, Nielsen, Rehman, Walther, Olsen, Mann: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, (2009) (PubMed).

Higuti, Tsurumi, Osaka, Kawamura, Tsujita, Yoshihara, Tani, Tanaka, Ichihara: "Molecular cloning of cDNA for the import precursor of human subunit B of H(+)-ATP synthase in mitochondria." in: Biochemical and biophysical research communications, Vol. 178, Issue 3, pp. 1014-20, (1991) (PubMed).

Target Details for ATP5F1

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: ATP5F1 (ATP5F1 Products)

Background: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) -containing the extramembraneous catalytic core, and F(0) -containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Molecular Weight: 28909

NCBI Accession: NP_001679

UniProt: P24539

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process