GFI1B antibody (C-Term)

Catalog No. ABIN1881370

This Rabbit Polyclonal antibody specifically detects GFI1B in WB. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for GFI1B antibody (C-Term) (ABIN1881370)

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFI1B antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB44051

Product Details anti-GFI1B Antibody

Binding Specificity: AA 302-330, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This GFI1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the C-terminal region of human GFI1B.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-GFI1B antibody (ABIN1881370)

Huang, Kuo, Lai, Suzuki, Sugano, Chang: "GATA-1 and NF-Y cooperate to mediate erythroid-specific transcription of Gfi-1B gene." in: Nucleic acids research, Vol. 32, Issue 13, pp. 3935-46, (2004) (PubMed).

Rödel, Wagner, Zörnig, Niessing, Möröy: "The human homologue (GFI1B) of the chicken GFI gene maps to chromosome 9q34.13-A locus frequently altered in hematopoietic diseases." in: Genomics, Vol. 54, Issue 3, pp. 580-2, (1999) (PubMed).

Target Details for GFI1B

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Alternative Name: GFI1B

Background: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Molecular Weight: 37492

NCBI Accession: NP_001128503, NP_004179

UniProt: Q5VTD9

Pathways: Cellular Response to Molecule of Bacterial Origin