MBD4 antibody (AA 323-351)

Catalog No. ABIN1881532

Product Details anti-MBD4 Antibody

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Binding Specificity: AA 323-351

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD4 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the Central region of human MBD4.

Clone: RB40399

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-MBD4 antibody (ABIN1881532)

Arora, Lindgren, Basu, Nagaraj, Gross, Weisdorf, Thyagarajan: "Polymorphisms in the base excision repair pathway and graft-versus-host disease." in: Leukemia, Vol. 24, Issue 8, pp. 1470-5, (2010) (PubMed).

Thyagarajan, Lindgren, Basu, Nagaraj, Gross, Weisdorf, Arora: "Association between genetic variants in the base excision repair pathway and outcomes after hematopoietic cell transplantations." in: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, Vol. 16, Issue 8, pp. 1084-9, (2010) (PubMed).

Liu, Wu, Chen, Ter-Minassian, Asomaning, Zhai, Wang, Su, Heist, Kulke, Lin, Liu, Christiani: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, (2010) (PubMed).

Target Details for MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Alternative Name: MBD4 (MBD4 Products)

Synonyms: MED1 antibody, Med1 antibody, methyl-CpG binding domain 4, DNA glycosylase antibody, methyl-CpG binding domain protein 4 antibody, methyl-CpG binding domain 4 DNA glycosylase antibody, MBD4 antibody, Mbd4 antibody

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.

Molecular Weight: 66051

NCBI Accession: NP_001263199, NP_001263201, NP_001263202, NP_003916

UniProt: O95243

Pathways: DNA Damage Repair