Nemo-Like Kinase antibody

Catalog No. ABIN1882269

Product Details anti-Nemo-Like Kinase Antibody

Target: Nemo-Like Kinase (NLK)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nemo-Like Kinase antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged NLK protein was used to produced this monoclonal antibody.

Clone: 1146CT24-2-1

Isotype: IgG2a

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Nemo-Like Kinase antibody (ABIN1882269)

Ohkawara, Shirakabe, Hyodo-Miura, Matsuo, Ueno, Matsumoto, Shibuya: "Role of the TAK1-NLK-STAT3 pathway in TGF-beta-mediated mesoderm induction." in: Genes & development, Vol. 18, Issue 4, pp. 381-6, (2004) (PubMed).

Ishitani, Kishida, Hyodo-Miura, Ueno, Yasuda, Waterman, Shibuya, Moon, Ninomiya-Tsuji, Matsumoto: "The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling." in: Molecular and cellular biology, Vol. 23, Issue 1, pp. 131-9, (2002) (PubMed).

Kehrer-Sawatzki, Moschgath, Maier, Legius, Elgar, Krone: "Characterization of the Fugu rubripes NLK and FN5 genes flanking the NF1 (Neurofibromatosis type 1) gene in the 5' direction and mapping of the human counterparts." in: Gene, Vol. 251, Issue 1, pp. 63-71, (2000) (PubMed).

Target Details for Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Alternative Name: NLK (NLK Products)

Synonyms: Amf antibody, Gpi1 antibody, AI194375 antibody, nlk antibody, nlkb antibody, zgc:111875 antibody, Xnlk antibody, nlk1 antibody, nlk2 antibody, NLK antibody, nemo-like antibody, RGD1561602 antibody, glucose-6-phosphate isomerase antibody, nemo like kinase antibody, nemo-like kinase, type 1 antibody, nemo-like kinase L homeolog antibody, Nemo-Like kinase antibody, nemo-like kinase antibody, Gpi antibody, NLK antibody, Nlk antibody, nlk1 antibody, nlk.L antibody, nlk antibody, nemo-like antibody

Background: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1.

Molecular Weight: 58283

NCBI Accession: NP_057315

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway