NPM1 antibody

Catalog No. ABIN1882271

Product Details anti-NPM1 Antibody

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged NPM1 protein was used to produced this monoclonal antibody.

Clone: 1042CT5-2-1

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-NPM1 antibody (ABIN1882271)

Bolli, Galimberti, Martelli, Tabarrini, Roti, Mecucci, Martelli, Petrini, Falini: "Cytoplasmic nucleophosmin in myeloid sarcoma occurring 20 years after diagnosis of acute myeloid leukaemia." in: The lancet oncology, Vol. 7, Issue 4, pp. 350-2, (2006) (PubMed).

Swaminathan, Kishore, Febitha, Kundu: "Human histone chaperone nucleophosmin enhances acetylation-dependent chromatin transcription." in: Molecular and cellular biology, Vol. 25, Issue 17, pp. 7534-45, (2005) (PubMed).

Tago, Chiocca, Sherr: "Sumoylation induced by the Arf tumor suppressor: a p53-independent function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 21, pp. 7689-94, (2005) (PubMed).

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1 (NPM1 Products)

Synonyms: B23 antibody, NPM antibody, NO38 antibody, Npm antibody, B23NP antibody, npm4 antibody, npm antibody, CG7917 antibody, CRP1 antibody, Crp1 antibody, Dmel\\CG7917 antibody, dNLP antibody, dNLP-S antibody, p22 antibody, npm1 antibody, sb:cb487 antibody, nucleophosmin 1 antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog antibody, nucleophosmin 1b antibody, Nucleophosmin antibody, nucleophosmin antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) antibody, Nucleoplasmin antibody, nucleophosmin 1a antibody, NPM1 antibody, Npm1 antibody, npm1.L antibody, npm1b antibody, npm antibody, Nlp antibody, npm1a antibody

Background: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication.

Molecular Weight: 32575

NCBI Accession: NP_001032827, NP_002511, NP_954654

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly