VRK1 antibody

Catalog No. ABIN1882291

Product Details anti-VRK1 Antibody

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VRK1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: Purified His-tagged VRK1 protein was used to produced this monoclonal antibody.

Clone: 1015CT2-1-1

Isotype: IgG1

Application Details

Application Notes: WB: 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-VRK1 antibody (ABIN1882291)

Blanco, Klimcakova, Vega, Lazo: "The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines." in: The FEBS journal, Vol. 273, Issue 11, pp. 2487-504, (2006) (PubMed).

Nichols, Traktman: "Characterization of three paralogous members of the Mammalian vaccinia related kinase family." in: The Journal of biological chemistry, Vol. 279, Issue 9, pp. 7934-46, (2004) (PubMed).

Barcia, López-Borges, Vega, Lazo: "Kinetic properties of p53 phosphorylation by the human vaccinia-related kinase 1." in: Archives of biochemistry and biophysics, Vol. 399, Issue 1, pp. 1-5, (2002) (PubMed).

Lopez-Borges, Lazo: "The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein." in: Oncogene, Vol. 19, Issue 32, pp. 3656-64, (2000) (PubMed).

Nezu, Oku, Jones, Shimane: "Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase." in: Genomics, Vol. 45, Issue 2, pp. 327-31, (1998) (PubMed).

Target Details for VRK1

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Alternative Name: VRK1 (VRK1 Products)

Synonyms: vrk1 antibody, MGC64390 antibody, MGC75762 antibody, VRK1 antibody, PCH1 antibody, PCH1A antibody, 51PK antibody, wu:fe16f06 antibody, zgc:56266 antibody, vaccinia related kinase 1 L homeolog antibody, vaccinia related kinase 1 antibody, Serine/threonine-protein kinase VRK1 antibody, vrk1.L antibody, vrk1 antibody, VRK1 antibody, vrk-1 antibody, Vrk1 antibody

Background: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm.

Molecular Weight: 45476

NCBI Accession: NP_003375

UniProt: Q99986