BUB3 antibody (C-Term)

Catalog No. ABIN1887091

Product Details anti-BUB3 Antibody

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Purification: Affinity chromatography purified via peptide column

Immunogen: 16 amino acid peptide from near the carboxy terminus of human bub3.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freezing and thawing repeatly.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term use.Store at -20 °C for long term preservation.

References for anti-BUB3 antibody (ABIN1887091)

Jiang, Li, Yang, Hawke, Zheng, Xia, Aldape, Wei, Guo, Chen, Lu: "PKM2 regulates chromosome segregation and mitosis progression of tumor cells." in: Molecular cell, Vol. 53, Issue 1, pp. 75-87, (2014) (PubMed).

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: Bub3 (BUB3 Products)

Synonyms: BUB3L antibody, hBUB3 antibody, Aa2-050 antibody, xbub3 antibody, AU019800 antibody, AU021329 antibody, AU043350 antibody, AW146323 antibody, C78067 antibody, BUB3, mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein antibody, BUB3 mitotic checkpoint protein L homeolog antibody, BUB3 antibody, Bub3 antibody, bub3.L antibody

Background: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis.Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy.Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function.Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3.Both Bub1 and Bub3 are mutually dependent for function.Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
Synonyms: Budding uninhibited by benzimidazoles 3, Bub3L

UniProt: O43684

Pathways: Maintenance of Protein Location