Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Cross-Reactivity (Details)
At least two isoforms of NIPSNAP3A are known to exist. NIPSNAP3A antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.
Purification
Affinity chromatography purified via peptide column
Immunogen
16 amino acid peptide near the center of human NIPSNAP3A.
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid freezing and thawing repeatly.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C for short term use.Store at -20 °C for long term preservation.
The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C.elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein.NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors.While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low.NIPSNAP3A protein is associated with plasma membrane and partially localized in rafts.NIPSNAP proteins have been suggested to be important in vesicular transport. Synonyms: NIPSNAP3A, Non-neuronal SNAP25-like protein 3A, NIPSNAP4, TASSC