HMHA1 antibody (AA 57-71)

Catalog No. ABIN1902592

Product Details anti-HMHA1 Antibody

Target: HMHA1 (Histocompatibility (Minor) HA-1 (HMHA1))

Binding Specificity: AA 57-71

Reactivity: Human, Cow, Dog, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This HMHA1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HMHA1 / HA-1. To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and will not recognise the peptide found eluted from HLA-A*0201 Molecules by den Haan et al (Science. 1998 Feb 13,279(5353):1054-7, PMID: 9461441)

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide KATGTLKRPTSLSRH-C from the N-terminus of human HMHA1 / HA-1 (NP_036424.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Hamster, Panda, Bovine, Dog, Porcine (100%), Mouse, Rat (93%), Opossum, Turkey, Chicken (87%), Zebra finch (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 200kD band in lysates of cell lines Jurkat and Molt4 after 0.05 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 125kD according to NP_036424.2. The 200kD band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HMHA1

Target: HMHA1 (Histocompatibility (Minor) HA-1 (HMHA1))

Alternative Name: HMHA1 / HA1 (HMHA1 Products)

Synonyms: RGD1308662 antibody, MGC80108 antibody, hmha1 antibody, arhgap45 antibody, ha-1 antibody, HMHA1 antibody, DKFZp469A2018 antibody, ARHGAP45 antibody, HA-1 antibody, HLA-HA1 antibody, 6330406L22Rik antibody, AW539505 antibody, Ha-1 antibody, Rho GTPase activating protein 45 antibody, Rho GTPase activating protein 45 L homeolog antibody, histocompatibility (minor) HA-1 b antibody, Arhgap45 antibody, ARHGAP45 antibody, arhgap45.L antibody, hmha1b antibody, arhgap45 antibody

Background: Name/Gene ID: ARHGAP45

Synonyms: ARHGAP45, HLA-HA1, HMHA1, Ha-1, KIAA0223, HA1

Gene ID: 23526

NCBI Accession: NP_036424