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GABPB2 antibody (Internal Region)

Verified GABPB2 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN190778
  • Target See all GABPB2 Antibodies
    GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))
    Binding Specificity
    • 7
    • 6
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 40
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 36
    • 4
    • 1
    Goat
    Clonality
    • 37
    • 3
    Polyclonal
    Conjugate
    • 21
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GABPB2 antibody is un-conjugated
    Application
    • 30
    • 26
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    GABPB2
    Sequence
    SSENSSKATD ETG
    Cross-Reactivity
    Dog, Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2, NP_002032.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 45+38 kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5 kDa according to NP_005245.2, NP_001307844.1 and of 38.1 kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 1

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))
    Alternative Name
    GABPB2 (GABPB2 Products)
    Synonyms
    GABP2 antibody, GABPB1 antibody, 1810015F01Rik antibody, 5830427M07 antibody, 9430006E19Rik antibody, A430024B14Rik antibody, AV050852 antibody, Gabpb2-1 antibody, RGD1565234 antibody, gabpb2 antibody, wu:fi15e02 antibody, wu:fi17h09 antibody, zgc:55805 antibody, ankhd1 antibody, GABPB2 antibody, GABPAP antibody, im:7142141 antibody, si:dkey-77n11.3 antibody, zgc:154003 antibody, GA binding protein transcription factor beta subunit 2 antibody, GA repeat binding protein, beta 2 antibody, GA binding protein transcription factor, beta subunit 2 antibody, GA binding protein transcription factor, beta subunit 2a antibody, GA binding protein transcription factor beta subunit 2 S homeolog antibody, GA binding protein transcription factor beta subunit 1 antibody, GA binding protein transcription factor, beta subunit 2b antibody, GABPB2 antibody, Gabpb2 antibody, gabpb2a antibody, gabpb2.S antibody, GABPB1 antibody, gabpb2b antibody
    Background
    GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53 kDa, GA binding protein transcription factor, beta subuni
    Gene ID
    2553
    NCBI Accession
    NP_005245, NP_002032, NP_001307844
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