NR0B1 antibody (Internal Region)

Catalog No. ABIN190789

Product Details anti-NR0B1 Antibody

Target: NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR0B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: DAX1 / NR0B1

Sequence: CGEDHPQQGS TLY

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CGEDHPQQGSTLY, from the internal region of the protein sequence according to NP_000466.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Adrenal Gland lysates (calculated MW of 51.7 kDa according to NP_000466.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR0B1

Target: NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))

Alternative Name: NR0B1 (NR0B1 Products)

Synonyms: DAX antibody, NR0B1 antibody, dax1 antibody, AHC antibody, AHCH antibody, AHX antibody, DAX-1 antibody, DAX1 antibody, DSS antibody, GTD antibody, HHG antibody, NROB1 antibody, SRXY2 antibody, Ahc antibody, Ahch antibody, Dax1 antibody, nuclear receptor subfamily 0 group B member 1 antibody, nuclear receptor subfamily 0, group B, member 1 antibody, nuclear receptor subfamily 0 group B member 1 L homeolog antibody, NR0B1 antibody, nr0b1 antibody, nr0b1.L antibody, Nr0b1 antibody

Background: NR0B1, DAX1, nuclear receptor subfamily 0, group B, member 1, AHC, AHCH, AHX, DAX-1, DSS, GTD, HHG, NROB1 , dosage-sensitive sex reversal, gonadotropin deficiency, nuclear hormone receptor

Gene ID: 190

NCBI Accession: NP_000466

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling