TCF3 antibody (Internal Region)

Catalog No. ABIN190880

Product Details anti-TCF3 Antibody

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TCF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: TCF3 / ITF1

Sequence: KAPRARTSPD EDED

Specificity: This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KAPRARTSPDEDED, from the internal region of the protein sequence according to NP_003191.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Daudi (calculated MW of 67.6 kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Alternative Name: TCF3 (TCF3 Products)

Synonyms: E2A antibody, E47 antibody, ITF1 antibody, TCF-3 antibody, VDIR antibody, bHLHb21 antibody, tcf3a antibody, zgc:113045 antibody, A1 antibody, AA408400 antibody, ALF2 antibody, AW209082 antibody, E12 antibody, E12/E47 antibody, ME2 antibody, Pan1 antibody, Pan2 antibody, Tcfe2a antibody, bhlhb21 antibody, e2a antibody, itf1 antibody, tcfe2a antibody, vdir antibody, xtcf-3 antibody, xtcf3 antibody, G12 antibody, TCF7L1 antibody, XE3 antibody, transcription factor 3 antibody, transcription factor 3b antibody, transcription factor 3 L homeolog antibody, transcription factor E2a antibody, TCF3 antibody, tcf3b antibody, Tcf3 antibody, tcf3.L antibody, tcfe2a antibody

Background: TCF3, ITF1, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), E2A, MGC129647, MGC129648 , E2A immunoglobulin enhancer-binding factor E12/E47, immunoglobulin transcription factor 1, kappa-E2-binding factor, transcription factor

Gene ID: 6929

NCBI Accession: NP_003191

Pathways: WNT Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response