STAT3 antibody (pTyr705)

Catalog No. ABIN196690

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: pTyr705

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. STAT3 (phospho-Tyr705) antibody detects endogenous levels of STAT3 only when phosphorylated at tyrosine 705.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of tyrosine 705 (A-P-YP-L-K).

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

References for anti-STAT3 antibody (ABIN196690)

Mpousis, Thysiadis, Avramidis, Katsamakas, Efthimiopoulos, Sarli: "Synthesis and evaluation of gallocyanine dyes as potential agents for the treatment of Alzheimer's disease and related neurodegenerative tauopathies." in: European journal of medicinal chemistry, Vol. 108, pp. 28-38, (2016) (PubMed).

Sangaletti, Tripodo, Sandri, Torselli, Vitali, Ratti, Botti, Burocchi, Porcasi, Tomirotti, Colombo, Chiodoni: "Osteopontin shapes immunosuppression in the metastatic niche." in: Cancer research, Vol. 74, Issue 17, pp. 4706-19, (2015) (PubMed).

Ma, Li, Wang, Gong, Zhang, Li, Ji, Liu, Chen, Xiang: "Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K-Akt signaling pathways." in: Regulatory peptides, Vol. 181, pp. 9-16, (2013) (PubMed).

Wang, Peng-Jiao, Yang, Chuang-Yuan, Zhao, Liu, Fei, Zhang: "PF-04691502 triggers cell cycle arrest, apoptosis and inhibits the angiogenesis in hepatocellular carcinoma cells." in: Toxicology letters, Vol. 220, Issue 2, pp. 150-6, (2013) (PubMed).

García-Prieto, González-López, Cabrera, Astudillo, Gutiérrez-Fernández, Fanjul-Fernandez, Batalla-Solís, Puente, Fueyo, López-Otín, Albaiceta: "Resistance to bleomycin-induced lung fibrosis in MMP-8 deficient mice is mediated by interleukin-10." in: PLoS ONE, Vol. 5, Issue 10, pp. e13242, (2010) (PubMed).

Takeichi, Sugiura, Muro, Matsumoto, Ogawa, Futamura, Kaminuma, Hashimoto, Shimoyama, Saito, Tomita: "Overexpression of LEDGF/DFS70 induces IL-6 via p38 activation in HaCaT cells, similar to that seen in the psoriatic condition." in: The Journal of investigative dermatology, Vol. 130, Issue 12, pp. 2760-7, (2010) (PubMed).

Zhang, Zhao, Xin: "Significance and relationship between Cripto-1 and p-STAT3 expression in gastric cancer and precancerous lesions." in: World journal of gastroenterology : WJG, Vol. 16, Issue 5, pp. 571-7, (2010) (PubMed).

Yuan, Qian, Shi, Lu, Li, Hu, Yu, Shen, Guo: "HER2-dependent MMP-7 expression is mediated by activated STAT3." in: Cellular signalling, Vol. 20, Issue 7, pp. 1284-91, (2008) (PubMed).

Yamaguchi, Zhu, Yu, Sasaki, Umetsu, Kidachi, Ryoyama: "Low-level bisphenol A increases production of glial fibrillary acidic protein in differentiating astrocyte progenitor cells through excessive STAT3 and Smad1 activation." in: Toxicology, Vol. 226, Issue 2-3, pp. 131-42, (2006) (PubMed).

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3 (STAT3 Products)

Synonyms: 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody

Background: Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3

Gene ID: 6774

NCBI Accession: NP_003141

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor