His Tag antibody

Details for Product No. ABIN1981363, Supplier: Log in to see
Antigen
Reactivity
Tag
625
28
15
3
3
Host
Mouse
414
187
27
23
8
8
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
58
46
36
17
14
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
564
402
164
108
79
78
75
26
13
11
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Purpose Monoclonal Antibody to detect His-taaged proteins
Immunogen 6X-HIS-KLH
Sequence HHHHHH
Clone 11E12
Isotype IgG1 kappa
Specificity Monoclonal Antibody detects His-taaged proteins
Characteristics Monoclonal Antibody to detect His-taaged proteins
Purification Protein A chromatography from in vitro production
Alternative Name His-Tag
Target Type Tag
Background A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Synonyms: His-Tag
Research Area Tags/Labels
Application Notes ELISA, WB
Comment

Suggested positive control:
Western blot: His-Tagged protein

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1 mg/mL in 0.15 M PBS
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN1981363) anti-His Tag antibody
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