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C5AR1 antibody (AA 15-27) (PE)

C5AR1 Reactivity: Human, Cow, Rabbit, Ferret FACS Host: Mouse Monoclonal S5-1 PE
Catalog No. ABIN1981892
  • Target See all C5AR1 Antibodies
    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
    Binding Specificity
    • 16
    • 15
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 15-27
    Reactivity
    • 109
    • 19
    • 18
    • 16
    • 13
    • 9
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Cow, Rabbit, Ferret
    Host
    • 79
    • 28
    • 17
    • 1
    • 1
    Mouse
    Clonality
    • 79
    • 42
    • 2
    Monoclonal
    Conjugate
    • 60
    • 11
    • 11
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This C5AR1 antibody is conjugated to PE
    Application
    • 70
    • 60
    • 37
    • 18
    • 18
    • 14
    • 13
    • 13
    • 8
    • 8
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody S5/1 recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade.
    Cross-Reactivity (Details)
    Human, Bovine, Ferret, Rabbit
    Purification
    Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR
    Clone
    S5-1
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product C5AR1 Primary Antibody
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze. Avoid prolonged exposure to light.
    Do not use after expiration date stamped on vial label.
    Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Camous, Roumenina, Bigot, Brachemi, Frémeaux-Bacchi, Lesavre, Halbwachs-Mecarelli: "Complement alternative pathway acts as a positive feedback amplification of neutrophil activation." in: Blood, Vol. 117, Issue 4, pp. 1340-9, (2011) (PubMed).

    Conway Morris, Kefala, Wilkinson, Dhaliwal, Farrell, Walsh, Mackenzie, Reid, Davidson, Haslett, Rossi, Sallenave, Simpson: "C5a mediates peripheral blood neutrophil dysfunction in critically ill patients." in: American journal of respiratory and critical care medicine, Vol. 180, Issue 1, pp. 19-28, (2009) (PubMed).

    Cain, Monk: "The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg(74)." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7165-9, (2002) (PubMed).

    Kiener, Baghestanian, Dominkus, Walchshofer, Ghannadan, Willheim, Sillaber, Graninger, Smolen, Valent: "Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis." in: Arthritis and rheumatism, Vol. 41, Issue 2, pp. 233-45, (1998) (PubMed).

  • Target
    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
    Alternative Name
    CD88 / C5aR (C5AR1 Products)
    Synonyms
    C5A antibody, C5AR antibody, C5R1 antibody, CD88 antibody, C5aR antibody, C5r1 antibody, Cd88 antibody, D7Msu1 antibody, GPR77 antibody, complement C5a receptor 1 antibody, complement component 5a receptor 1 antibody, C5AR1 antibody, C5ar1 antibody, c5ar1 antibody
    Background
    Complement C5a receptor 1,CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.,C5A, C5R1, C5AR1
    Gene ID
    728
    UniProt
    P21730
    Pathways
    Complement System
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