serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1) (AA 1-433) antibody

Details for Product No. ABIN1999238
Antigen
Epitope
AA 1-433
10
8
8
7
6
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
88
29
25
6
4
4
4
4
3
2
1
Host
Rabbit
69
19
1
Clonality
Polyclonal
Conjugate
Un-conjugated
6
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
60
32
20
13
12
10
6
1
1
1
1
Options
Immunogen Recombinant Human HAI-1 / SPINT1 protein (ABIN2004743)
Isotype IgG
Specificity Human HAI-1 / SPINT1
Characteristics Produced in rabbits immunized with purified, recombinant Human HAI-1 / SPINT1 (rh HAI-1 / SPINT1, ABIN2004743 O43278-2, Met 1-Val 433,. Total IgG was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Antigen
Alternative Name SPINT1 (SPINT1 Antibody Abstract)
Background Kunitz-type protease inhibitor 1, also known as hepatocyte growth factor activator inhibitor type 1, HAI-1 and SPINT1, is a secreted protein which belongs to the Kunitz family of serine protease inhibitors. SPINT1 / HAI-1 contains twoBPTI / Kunitz inhibitor domains, oneLDL- receptor class A domain and oneMANSC domain. SPINT1 / HAI-1 is an epithelial Kunitz- type transmembrane serine protease inhibitor that is encoded by the SPINT1 gene. SPINT1 / HAI-1 is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. SPINT1 / HAI-1 is a membrane-bound, serine proteinase inhibitor initially identified as an inhibitor of hepatocyte growth factor activator. It displays potent inhibitory activity toward a large number of trypsin-like serine proteases. SPINT1 / HAI-1 is shown to play an essential role in postnatal epithelial homeostasis. SPINT1 / HAI-1 has critical roles in the regulated keratinization of the epidermis and hair development. It inhibits matriptase and prostasin, both of which are membrane-bound serine proteinases that have critical roles in epidermal differentiation and function. Matriptase suppression is an essential function of SPINT1 / HAI-1 in postnatal tissue homeostasis.
UniProt O43278-2
Application Notes WB: 10-20 μg/mL
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human SPINT1. The detection limit for Human SPINT1 is approximately 0.00245 ng/well.
Restrictions For Research Use only
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months