CLIC4 antibody (Chloride Intracellular Channel 4) (AA 2-253)

Details for Product anti-CLIC4 Antibody No. ABIN1999651, Supplier: Log in to see
Antigen
  • CLIC4L
  • H1
  • MTCLIC
  • huH1
  • p64H1
  • cb142
  • wu:fb33c05
  • wu:fc30h10
  • clic4
  • MGC89006
  • CLIC4
  • DKFZp459A0325
  • D0Jmb3
  • TU-74
  • mc3s5
  • mtCLIC
  • xclic4
  • chloride intracellular channel 4
  • chloride intracellular channel 4 (mitochondrial)
  • chloride intracellular channel 4 S homeolog
  • CLIC4
  • Clic4
  • clic4
  • clic4.S
Epitope
AA 2-253
26
15
7
2
2
2
2
1
Reactivity
Human
105
70
61
4
4
3
1
1
1
1
1
Host
Rabbit
92
21
6
Clonality
Polyclonal
Conjugate
This CLIC4 antibody is un-conjugated
11
5
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
104
56
51
26
25
13
8
8
4
3
Options
Supplier
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Immunogen Recombinant Human CLIC4 protein (ABIN2005158)
Isotype IgG
Specificity Human CLIC4
Characteristics Produced in rabbits immunized with purified, recombinant Human CLIC4 (rh CLIC4, ABIN2005158 Q9Y696-1, Ala 2-Lys 253,. CLIC4 specific IgG was purified by Human CLIC4 affinity chromatography.
Purification purified by affinity chromatography
Sterility 0.2 μm filtered
Plasmids, Primers & others Plasmids, Primers & others CLIC4 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CLIC4 (CLIC4 Antibody Abstract)
Background Chloride intracellular channel protein 4, also known as Intracellular chloride ion channel protein p64H1 and CLIC4, is a member of the chloride channel CLIC family. It contains one,GST C-terminal domain. CLIC4 is a member of a family of intracellular chloride channels. It is regulated by p53, c-Myc, and tumor necrosis factor-alpha. CLIC4 is detected in epithelial cells from colon, esophagus and kidney (at protein level). CLIC4 has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. CLIC4 could promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Expression of CLIC4 is prominent in heart, kidney, placenta and skeletal muscle. Overexpression of CLIC4 in cancer cells inhibits tumor growth. Conversely, overexpression of CLIC4 in tumor stromal cells stimulates tumor growth. Thus, CLIC4 participates in normal and pathological processes and may serve as a useful target for therapies in disturbances of homeostasis and neoplastic transformation. Loss of CLIC4 in tumor cells and gain in tumor stroma is common to many human cancers and marks malignant progression. Up-regulation of CLIC4 in tumor stroma is coincident with myofibroblast conversion, generally a poor prognostic indicator. Reactivation and restoration of CLIC4 in tumor cells or the converse in tumor stromal cells could provide a novel approach to inhibit tumor growth.
UniProt Q9Y696-1
Application Notes WB: 2-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CLIC4. The detection limit for Human CLIC4 is approximately 0.039 ng/well.
IP: 1-4 μg/mg of lysate
Restrictions For Research Use only
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Chloride Intracellular Channel 4 (CLIC4) (AA 2-253) antibody (ABIN1999651) anti-Chloride Intracellular Channel 4 (CLIC4) (AA 2-253) antibody
Immunoprecipitation (IP) image for anti-Chloride Intracellular Channel 4 (CLIC4) (AA 2-253) antibody (ABIN1999651) anti-Chloride Intracellular Channel 4 (CLIC4) (AA 2-253) antibody (Image 2)
Background publications Suh, Mutoh, Mutoh, Li, Ryscavage, Crutchley, Dumont, Cheng, Yuspa: "CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation." in: Journal of cell science, Vol. 120, Issue Pt 15, pp. 2631-40, 2007 (PubMed).

Berryman, Goldenring: "CLIC4 is enriched at cell-cell junctions and colocalizes with AKAP350 at the centrosome and midbody of cultured mammalian cells." in: Cell motility and the cytoskeleton, Vol. 56, Issue 3, pp. 159-72, 2003 (PubMed).

Rønnov-Jessen, Villadsen, Edwards, Petersen: "Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts." in: The American journal of pathology, Vol. 161, Issue 2, pp. 471-80, 2002 (PubMed).

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