Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 1-251) antibody

Details for Product No. ABIN1999925
Antigen
  • CD142
  • TF
  • TFA
  • f3
  • AA409063
  • Cf-3
  • Cf3
  • tf
  • zgc:112151
  • coagulation factor III, tissue factor
  • coagulation factor IIIa
  • coagulation factor III
  • tissue factor
  • coagulation factor IIIb
  • coagulation factor III (thromboplastin, tissue factor) S homeolog
  • F3
  • f3a
  • tf
  • f3b
  • f3.S
Epitope
AA 1-251
16
15
12
10
9
9
8
6
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
182
56
49
21
20
3
3
2
1
Host
Mouse
150
72
12
9
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
28
26
6
5
4
3
3
3
3
3
3
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
164
88
75
63
20
20
20
16
13
13
13
4
4
4
3
2
2
1
1
1
Options
Immunogen Recombinant Human F3 / Tissue factor / CD142 protein (ABIN2005478)
Clone 05
Isotype IgG2a
Specificity Human F3 / Tissue factor / CD142
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
No Cross-Reactivity Human
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human F3 / Tissue factor / CD142 (rh F3 / Tissue factor / CD142, ABIN2005478 P13726, Met 1-Glu 251,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Antigen
Alternative Name F3 (F3 Antibody Abstract)
Background Tissue factor, also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Because of its essential role in hemostasis, Tissue factor plays a role in pathology associated with hemostasis, triggering the coagulation system in many thrombotic diseases and the coagulopathies associated with sepsis and other forms of disseminated intravascular coagulation. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation, the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes.
UniProt P13726
Pathways Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human F3. The detection limit for Human F3 is approximately 0.16 ng/well.
ICC/IF: 10-25 μg/mL
FCM: 0.5-2 μg/Test
Restrictions For Research Use only
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 1-251) antibody (ABIN1999925) Immunofluorescence staining of Human F3 in A431 cells. Cells were fixed with 4% PFA, ...
Image no. 2 for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 1-251) antibody (ABIN1999925) Flow cytometric analysis of Human F3(CD142) expression on A431 cells. Cells were stai...
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