BLMH antibody (Bleomycin Hydrolase) (AA 2-455)

Details for Product anti-BLMH Antibody No. ABIN2001299
Antigen
  • AI035728
  • Bh
  • Bmh
  • wu:fb13c01
  • zgc:66261
  • bh
  • bmh
  • BH
  • BMH
  • bleomycin hydrolase
  • Bleomycin hydrolase
  • bleomycin hydrolase S homeolog
  • BLMH
  • pepC
  • LOC5569945
  • Apre_0140
  • Apar_0899
  • Smon_1289
  • Kfla_1242
  • Snas_3912
  • BLJ_1941
  • Olsu_1085
  • PGTDC60_0106
  • Blmh
  • blmh
  • blmh.S
Epitope
AA 2-455
9
6
4
4
3
3
3
2
2
1
1
1
1
1
Reactivity
Mouse (Murine)
72
43
32
4
4
4
3
3
2
2
1
1
1
Host
Rabbit
69
18
1
Clonality
Polyclonal
Conjugate
This BLMH antibody is un-conjugated
9
5
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
61
28
20
13
11
10
6
6
5
2
2
1
Options
Immunogen Recombinant Mouse BLMH / Bleomycin Hydrolase protein (ABIN2008125)
Isotype IgG
Specificity Mouse BLMH / Bleomycin Hydrolase
Characteristics Produced in rabbits immunized with purified, recombinant Mouse BLMH / Bleomycin Hydrolase (rM BLMH / Bleomycin Hydrolase, ABIN2008125 NP_848760.1, Asn 2-Glu 455,. BLMH / Bleomycin Hydrolase specific IgG was purified by Mouse BLMH / Bleomycin Hydrolase affinity chromatography.
Purification purified by affinity chromatography
Sterility 0.2 μm filtered
Plasmids, Primers & others Plasmids, Primers & others BLMH products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name BLMH (BLMH Antibody Abstract)
Background The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM- induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
NCBI Accession NP_848760.1
Application Notes WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse BLMH. The detection limit for Mouse BLMH is approximately 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
Restrictions For Research Use only
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months
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