PIK3R1 antibody (AA 600-650)

Catalog No. ABIN2017599

Product Details anti-PIK3R1 Antibody

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Binding Specificity: AA 600-650

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: PI3 Kinase p85 alpha Antibody detects endogenous levels of human PI3 Kinase p85 alpha. Predicted to react with mouse and rat PI3 Kinase p85 alpha according to sequence homology.Positive Control: Ramos

Cross-Reactivity (Details): PI3 Kinase p85 alpha Antibody detects endogenous levels of human PI3 Kinase p85 alpha. Predicted to react with mouse and rat PI3 Kinase p85 alpha according to sequence homology.Positive Control: Ramos

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 600-650 of human PI3 Kinase p85 alpha .

Isotype: IgG2b

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 2-4 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther Applications: user optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PI3 Kinase p85 alpha (PI3K p85a Products)

Synonyms: PI3CG antibody, PI3K antibody, PI3Kgamma antibody, PIK3 antibody, AGM7 antibody, GRB1 antibody, p85 antibody, p85-ALPHA antibody, PI3KA antibody, AA414921 antibody, C530050K14 antibody, p50alpha antibody, p55alpha antibody, p85alpha antibody, wu:fb65b05 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma antibody, phosphoinositide-3-kinase regulatory subunit 1 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta antibody, phosphoinositide-3-kinase, regulatory subunit 1 (alpha) antibody, PIK3CG antibody, PIK3R1 antibody, Pik3r1 antibody, Pik3cb antibody, pik3r1 antibody

Background: Phosphoinositide 3-kinase (PI3K) catalyzes the phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit (p85). The catalytic subunits of PI3K include p110 alpha, p110 beta, p110 gamma, and p110 delta, while the regulatory subunits contain p85 alpha and p85 beta. p85 alpha regulates the catalytic activity of p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain. The p85 alpha has been implicated in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.PI3 Kinase p85 alpha Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 600-50 of human PI3 Kinase p85 alpha (Swiss Prot: P27986).

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect