HLAG antibody

Catalog No. ABIN206902

Product Details anti-HLAG Antibody

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLAG antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form

Purification: Precipitation

Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Type of Immunogen: Recombinant protein

Clone: MEM-G-9

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 5 μg/mL), Flo (2 - 5 μg/mL), ICC, IHC, IHC-Fr (2 - 5 μg/mL), IP (2 - 5 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 1-5 μg/mL. Positive control - JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution -. Alexa Fluor®. 488 Fab-fragment: 5 μg/mL. ELISA: Application note - The antibody MEM-G/9 has been t The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide free PBS, 0.3 M sodium chloride, preserved by filter sterilization, approx,  pH 7.4.

Preservative: Azide free

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G (HLAG Products)

Synonyms: MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody

Background: Name/Gene ID: HLA-G

Synonyms: HLA-G, HLAG, HLA-6.0, MHC class I antigen G, HLA G antigen

Gene ID: 3135

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints