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CD4 antibody (CD4 Molecule) (AA 1-390) (FITC) Primary Antibody

CD4 Reactivity: Human FACS Host: Mouse Monoclonal 08
Independent Validation (1)
Catalog No. ABIN2104933
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100 tests ABIN2104933
100 tests ABIN2104933
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  • Target
    Binding Specificity
    AA 1-390
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This CD4 antibody is conjugated to FITC
    Application
    Flow Cytometry (FACS)
    Specificity
    Human CD4
    Characteristics
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD4 (rh CD4, ABIN2002782 NP_000607.1, Met1-Trp390, and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
    Immunogen
    Recombinant Human CD4 protein (ABIN2002782)
    Clone
    08
    Isotype
    IgG1
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Validation #029847 (Flow Cytometry)
    'Independent Validation' Badge
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029847
    Date
    08/31/2015
    Antigen
    Lot Number
    HG08FE1721
    Method validated
    Flow Cytometry
    Positive Control
    peripheral blood mononuclear cells
    Negative Control
    peripheral blood mononuclear cells
    Notes
    This antibody performed well and is comparable to anti-CD4-FITC from BD Pharmingen (see data below)
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: human CD4 (CD4)
    • Catalog number: ABIN2104933
    • Lot number: HG08FE1721
    • Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA
    Secondary Antibody
    Full Protocol
    • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
    • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
    • 3. Spin for 20 minutes at 1500 rpm with break off.
    • 4. The buffy coat layer was collected and wash with PBS/BSA.
    • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
    • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
    • 7. 100ul of cells (1x10^6) were added to their respective tubes.
    • 8. Add 5µl of anti-CD4-FITC (Sino Biological) or 20µl of anti-CD4-FITC (BD Pharmingen) to the appropriate tubes and incubate for 20 minutes on ice.
    • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA.
    • 10. Analyze
    Experimental Notes
  • Format
    Liquid
    Concentration
    0.1 mg/mL
    Buffer
    Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
    Storage
    4 °C
    Storage Comment
    This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
    Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
    Expiry Date
    12 months
  • Target
    Alternative Name
    CD4 (CD4 Antibody Abstract)
    Synonyms
    cd4, L3T4, Ly-4, CD4mut, W3/25, p55, CD4 molecule, carcinoembryonic antigen-related cell adhesion molecule 5, T-cell surface glycoprotein CD4, CD4 antigen, Cd4 molecule, CD4, LOC100125525, cd4, Cd4
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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