RPS6KB1 antibody (AA 511-525)

Catalog No. ABIN211257

Product Details anti-RPS6KB1 Antibody

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Binding Specificity: AA 511-525

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Chicken, Pig, Monkey, Horse, Goat, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes rat p70 S6 kinase at 64kD. Species cross-reactivity: human and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Xenopus (93%) Stickleback, Zebrafish (87%).

Purification: Protein A purified

Immunogen: 16 residue synthetic peptide (C-FPMISKRPEHLRMNL) corresponding to residues 511-525 of rat S6 kinase. The immunizing peptide is identical to the human and rabbit sequence. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Xenopus (93%), Stickleback, Zebrafish (87%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2.0 μg/mL detected S6 Kinase in RIPA lysates from mouse 3T3 cells. Additional bands above 64kD may be observed at higher concentrations of the antibody. Previous lots detected S6 Kinase in RIPA lysates from human A431 and rat L6 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Not recommended.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: RPS6KB1 / P70S6K / S6K (RPS6KB1 Products)

Synonyms: PS6K antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, STK14A antibody, p70 S6KA antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, 10539 antibody, 7084 antibody, CG10539 antibody, DS6K antibody, Dmel\\CG10539 antibody, Dp70S6k antibody, Dp70[s6k] antibody, Dp70s6k antibody, S6k antibody, dS6K antibody, dS6k antibody, dp70[S6k] antibody, dp70s6k antibody, dps6k antibody, ds6k antibody, fs(3)07084 antibody, l(3)07084 antibody, p70 S6K antibody, p70/S6K antibody, p70S6K antibody, p70[S6 kinase] antibody, p70[S6K] antibody, p70[S6k] antibody, p70[S6kinase] antibody, p70s6K antibody, s6k antibody, s6k11 antibody, 2610318I15Rik antibody, 4732464A07Rik antibody, 70kDa antibody, AA959758 antibody, AI256796 antibody, AI314060 antibody, p70/85s6k antibody, p70s6k antibody, p70s6k-A antibody, p70-s6k antibody, ps6k antibody, rps6kb1 antibody, rps6kb1-A antibody, s6K1 antibody, stk14a antibody, fc51h01 antibody, wu:fc51h01 antibody, zgc:55713 antibody, ribosomal protein S6 kinase B1 antibody, Ribosomal protein S6 kinase antibody, ribosomal protein S6 kinase, polypeptide 1 antibody, ribosomal protein S6 kinase B1 L homeolog antibody, ribosomal protein S6 kinase B1 S homeolog antibody, ribosomal protein S6 kinase b, polypeptide 1b antibody, RPS6KB1 antibody, S6k antibody, Rps6kb1 antibody, rps6kb1.L antibody, rps6kb1.S antibody, rps6kb1b antibody

Background: Name/Gene ID: RPS6KB1
Subfamily: P70 RSK
Family: Protein Kinase

Synonyms: RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha

Gene ID: 6198

UniProt: P23443

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect