CHEK1 antibody (AA 194-446)

Catalog No. ABIN1858393

Product Details anti-CHEK1 Antibody

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Binding Specificity: AA 194-446

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Checkpoint Kinase 1 (CHEK1)

Specificity: The antibody is a rabbit polyclonal antibody raised against CHEK1. It has been selected for its ability to recognize CHEK1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Checkpoint Kinase 1 (CHEK1) corresdonding to Cys194~Gly446 (Accession # O35280)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:220-880 Immunohistochemistry: 5-20 μg/mL

1:22-88 Immunocytochemistry: 5-20 μg/mL

1:22-88 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Checkpoint Kinase 1 (CHEK1 Products)

Synonyms: An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody

Background: CHK1, Cell cycle checkpoint kinase, Serine/threonine-protein kinase Chk1

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair