NR5A2 + LRH1 antibody (Internal Region)

Catalog No. ABIN213385

This Rabbit Polyclonal antibody specifically detects NR5A2 + LRH1 in IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Dog, Horse, Zebrafish (Danio rerio), Monkey, Chicken, Hamster and Xenopus laevis.

Quick Overview for NR5A2 + LRH1 antibody (Internal Region) (ABIN213385)

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Reactivity: Human, Rat, Mouse, Dog, Horse, Zebrafish (Danio rerio), Monkey, Chicken, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR5A2 + LRH1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NR5A2 + LRH1 Antibody

Binding Specificity: Internal Region

Brand: IHC-plus™

Specificity: Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Elephant, Panda, Horse, Opossum, Turkey, Chicken, Platypus, Xenopus, Trout, Zebrafish, Stickleback (100%) Sheep, Bovine, Bat, Pufferfish (94%) Chimpanzee (88%) Catfish (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human NR5A2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Elephant, Panda, Horse, Opossum, Turkey, Chicken, Platypus, Xenopus, Trout, Zebrafish, Stickleback (100%), Sheep, Bovine, Bat, Pufferfish (94%), Chimpanzee (88%), Catfish (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 - 20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 - 5 μg/mL. Antibody does not work with tissues fixed in 4 % PFA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NR5A2 + LRH1

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Alternative Name: NR5A2 / LRH-1

Background: Name/Gene ID: NR5A2
Subfamily: NR5 Fushi tarazu-like
Family: NHR

Synonyms: NR5A2, B1F, B1F2, B1-binding factor, CPF, CYP7A promoter-binding factor, FTZ-F1beta, FTF, Liver receptor homolog 1, LRH1, LRH-1, HB1F, HB1F-2, Nuclear receptor phr-1, FTZ-F1, Liver receptor homolog-1, Nuclear receptor NR5A2, Receptor phr

Gene ID: 2494

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway