EMR3 antibody (C-Term)

Catalog No. ABIN213514

Product Details anti-EMR3 Antibody

Target: EMR3 (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 3 (EMR3))

Binding Specificity: C-Term

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EMR3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human EMR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Orangutan, Gibbon (94%) Elephant (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from C-terminal cytoplasmic domain of human EMR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Orangutan, Gibbon (94%), Elephant (89%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EMR3

Target: EMR3 (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 3 (EMR3))

Alternative Name: ADGRE3 / EMR3 (EMR3 Products)

Synonyms: EMR3 antibody, DKFZp459A1439 antibody, adhesion G protein-coupled receptor E3 antibody, ADGRE3 antibody, EMR3 antibody

Background: Name/Gene ID: ADGRE3
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRE3, EGF-like module receptor 3, EMR3

Gene ID: 84658