HUWE1 antibody (C-Term)

Catalog No. ABIN213933

Product Details anti-HUWE1 Antibody

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUWE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HUWE1

Target: HUWE1 (HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1))

Alternative Name: HUWE1 / ARFBP1 (HUWE1 Products)

Background: Name/Gene ID: HUWE1

Synonyms: HUWE1, ARF-binding protein 1, ARF-BP1, BJ-HCC-24 tumor antigen, Ib772, KIAA0312, LASU1, Mcl-1 ubiquitin ligase E3, HECTH9, MULE, HSPC272, KIAA1578, URE-B1, UREB1, URE-binding protein 1, HECT domain protein LASU1, Large structure of UREB1

Gene ID: 10075

UniProt: Q7Z6Z7