MYL9 antibody (AA 12-27)

Catalog No. ABIN214356

Product Details anti-MYL9 Antibody

Target: MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))

Binding Specificity: AA 12-27

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Amino acids 12-27 of human myosin light chain protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human MYL9.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 μg/mL), IP, WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MYL9

Target: MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))

Alternative Name: MLC2 / MYL9 (MYL9 Products)

Synonyms: LC20 antibody, MLC-2C antibody, MLC2 antibody, MRLC1 antibody, MYRL2 antibody, AI327049 antibody, MLC20 antibody, Mylc2c antibody, RLC-C antibody, myosin antibody, regulatory antibody, MRCL3 antibody, MRLC2 antibody, MYL9 antibody, zgc:103467 antibody, myl9 antibody, zgc:77916 antibody, myosin light chain 9 antibody, myosin, light polypeptide 9, regulatory antibody, myosin light chain 9 L homeolog antibody, myosin, light chain 9, regulatory antibody, myosin, light chain 12A, regulatory, non-sarcomeric antibody, myosin, light chain 9a, regulatory antibody, myosin, light chain 9b, regulatory antibody, MYL9 antibody, Myl9 antibody, myl9.L antibody, MYL12A antibody, myl9a antibody, myl9b antibody

Background: Name/Gene ID: MYL9
Family: Protein Kinase Non Catalytic

Synonyms: MYL9, 20 kDa myosin light chain, LC20, MLC-2C, MLC2, Myosin RLC, MRLC1, MYRL2

Gene ID: 10398

UniProt: P24844