MAD2L2 antibody (AA 3-14)

Catalog No. ABIN214364

Product Details anti-MAD2L2 Antibody

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Binding Specificity: AA 3-14

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2L2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Aa 3-14 of Human MAD2L2. MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human MAD2L2.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:7500 - 1:30000), IF, IHC, IHC-P (10 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MAD2L2

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Alternative Name: MAD2B / REV7 (MAD2L2 Products)

Synonyms: mad2b antibody, 2310033C13Rik antibody, MAD2B antibody, REV7 antibody, mad2B antibody, mad2l2-A antibody, xMAD2L2 antibody, POLZ2 antibody, mad2l2 antibody, zgc:110299 antibody, mitotic arrest deficient 2 like 2 antibody, MAD2 mitotic arrest deficient-like 2 antibody, mitotic arrest deficient 2 like 2 S homeolog antibody, MAD2 mitotic arrest deficient-like 2 (yeast) antibody, si:dkey-23c22.2 antibody, mad2l2 antibody, MAD2L2 antibody, Mad2l2 antibody, mad2l2.S antibody, si:dkey-23c22.2 antibody

Background: Name/Gene ID: MAD2L2

Synonyms: MAD2L2, POLZ2, REV7 homolog, REV7, MAD2-like protein 2, MAD2B, HREV7

Gene ID: 10459

UniProt: Q9UI95