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CD16 antibody (Biotin)

CD16 Reactivity: Human FACS Host: Mouse Monoclonal 4ACB16 Biotin
Catalog No. ABIN2144281
  • Target See all CD16 Antibodies
    CD16
    Reactivity
    • 128
    • 26
    • 20
    • 20
    • 20
    • 9
    • 8
    • 8
    • 7
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 111
    • 29
    • 14
    • 1
    Mouse
    Clonality
    • 127
    • 26
    • 1
    Monoclonal
    Conjugate
    • 56
    • 18
    • 13
    • 12
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD16 antibody is conjugated to Biotin
    Application
    • 125
    • 31
    • 28
    • 25
    • 24
    • 23
    • 21
    • 17
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS)
    Characteristics
    4ACB16 reacts with human CD16, a 50-65 kDa Fcg receptor IIIa (FcgRIII), expressed on NK cells, monocytes/macrophages and granulocytes.
    Clone
    4ACB16
    Isotype
    IgG1
    Top Product
    Discover our top product CD16 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Procedure
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    • Repeat step 5.
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    CD16
    Alternative Name
    CD16 (CD16 Products)
    Synonyms
    Fcgr3 antibody, Fcgr3a antibody, CD16 antibody, FCGR3 antibody, FCGR3@ antibody, CD16b antibody, FCG3 antibody, FCR-10 antibody, FCRIII antibody, FCRIIIb antibody, FcRIII antibody, Fc fragment of IgG, low affinity IIa, receptor antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody, Fc fragment of IgG receptor IIIb antibody, Fc fragment of IgG receptor IIIa antibody, Fc receptor, IgG, low affinity III antibody, Fc-gamma RIII antibody, Fcgr2a antibody, FCGR3A antibody, FCGR3B antibody, Fcgr3 antibody, FCGR3 antibody
    Background
    It is a human NK cell associated antigen. CD16 is a low affinity receptor for IgG which functions in phagocytosis and ADCC, as well as in signal transduction and NK cell activation. The CD16 antibody blocks the binding of soluble immune complexes to granulocytes.This clone is cross reactive with non-human primate.
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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