CTLA4 antibody (Cytotoxic T-Lymphocyte-Associated Protein 4) (FITC)

Details for Product anti-CTLA4 Antibody No. ABIN2144726
Antigen
  • CTLA-4
  • CTLA4
  • CD152
  • Cd152
  • Ctla-4
  • Ly-56
  • CD
  • CELIAC3
  • GRD4
  • GSE
  • IDDM12
  • sCTLA4
  • cytotoxic T-lymphocyte associated protein 4
  • cytotoxic T-lymphocyte-associated protein 4
  • CTLA4
  • Ctla4
Alternatives
anti-Human CTLA4 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
177
123
38
2
2
2
Host
Mouse
120
96
67
22
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CTLA4 antibody is conjugated to FITC
36
26
24
12
7
3
3
3
3
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS)
169
98
92
56
55
23
18
16
11
10
9
6
4
4
1
1
1
1
1
1
1
Options
Clone 4AA3-6B10
Isotype IgG2a
Characteristics CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. Regulatory T cells express high level of CTLA-4. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation.
Plasmids, Primers & others Plasmids, Primers & others CTLA4 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD152 (CTLA4 Antibody Abstract)
Pathways Cancer Immune Checkpoints
Application Notes Optimal working dilution should be determined by the investigator.
Assay Procedure
  • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
  • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
  • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
  • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
  • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
  • [Cell fixation: 2 % formaldehyde solution]
Restrictions For Research Use only
Format Liquid
Buffer Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
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