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CD58 antibody (PE)

CD58 Reactivity: Human FACS Host: Mouse Monoclonal 4ATS2-9-1-1-1 PE
Catalog No. ABIN2144939
  • Target See all CD58 Antibodies
    CD58
    Reactivity
    • 76
    • 5
    • 3
    • 2
    Human
    Host
    • 39
    • 37
    Mouse
    Clonality
    • 39
    • 37
    Monoclonal
    Conjugate
    • 30
    • 8
    • 8
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD58 antibody is conjugated to PE
    Application
    • 40
    • 33
    • 13
    • 13
    • 10
    • 7
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    Flow Cytometry (FACS)
    Characteristics
    4ATS2/9.1 reacts with 55-70 kDa CD58, lymphocyte function-associated antigen (LFA-3). It is expressed in hematipoietic and non-hematopoietic tissue including leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.CD58 mediates adhesion between killer and target cells, antigen presenting cells and T cells, thymocytes and thymic epithelial cells. It also provides co-stimulatory signal in immune response.
    Clone
    4ATS2-9-1-1-1
    Isotype
    IgG1
    Top Product
    Discover our top product CD58 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Procedure
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    CD58
    Alternative Name
    CD58 (CD58 Products)
    Synonyms
    LFA-3 antibody, LFA3 antibody, ag3 antibody, CD58 molecule antibody, CD58 antibody
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