Ret Proto-Oncogene antibody (Extracellular Domain)

Catalog No. ABIN2150516

Product Details anti-Ret Proto-Oncogene Antibody

Target: Ret Proto-Oncogene (RET)

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Ret Proto-Oncogene antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Purified

Immunogen: Ret antibody was raised in goat using NSO derived murine ret extracellular domain as the immunogen.

Application Details

Application Notes: ELISA: 0.5-1 μg/mL, IHC-F: 5 μg/mL, IHC-P: 10-15 μg/mL, WB: 0.1-0.2 μg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2 containing 5 % trehalose.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -80 °C

Storage Comment: Aliquot and store at -70 °C or lower.

References for anti-Ret Proto-Oncogene antibody (ABIN2150516)

Gay, Valenta, Herr, Paratore-Hari, Basler, Sommer: "Distinct adhesion-independent functions of β-catenin control stage-specific sensory neurogenesis and proliferation." in: BMC biology, Vol. 13, pp. 24, (2015) (PubMed).

Aron, Klein, Pham, Kramer, Wurst, Klein: "Pro-survival role for Parkinson's associated gene DJ-1 revealed in trophically impaired dopaminergic neurons." in: PLoS biology, Vol. 8, Issue 4, pp. e1000349, (2010) (PubMed).

Target Details for Ret Proto-Oncogene

Target: Ret Proto-Oncogene (RET)

Alternative Name: Ret (RET Products)

Synonyms: c-ret antibody, cret antibody, etID315074.13 antibody, ret1 antibody, wu:fd13h01 antibody, X-ret antibody, ret-A antibody, xret antibody, RET antibody, MTC1 antibody, CDHF12 antibody, CDHR16 antibody, HSCR1 antibody, MEN2A antibody, MEN2B antibody, PTC antibody, RET-ELE1 antibody, RET51 antibody, RET9 antibody, c-Ret antibody, ret proto-oncogene receptor tyrosine kinase antibody, ret proto-oncogene S homeolog antibody, ret proto-oncogene antibody, ret antibody, ret.S antibody, RET antibody, Ret antibody

Background: The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation