HIF1AN antibody (AA 183-232)

Catalog No. ABIN6736017

Product Details anti-HIF1AN Antibody

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Binding Specificity: AA 183-232

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Horse, Cow, Guinea Pig, Zebrafish (Danio rerio), Pig, Monkey, Chicken, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1AN antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human HIF1AN

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa183-232 of human HIF1AN (Q9NWT6, NP_060372). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Trout, Catfish, Stickleback, Pike, Zebrafish (100%), Beetle (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 2.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HIF1AN

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Alternative Name: HIF1AN (HIF1AN Products)

Synonyms: FIH1 antibody, 2310046M24Rik antibody, A830014H24Rik antibody, cb728 antibody, id:ibd5139 antibody, hypoxia inducible factor 1 alpha subunit inhibitor antibody, hypoxia-inducible factor 1, alpha subunit inhibitor antibody, hypoxia inducible factor 1, alpha subunit inhibitor antibody, hypoxia-inducible factor 1, alpha subunit inhibitor S homeolog antibody, HIF1AN antibody, Hif1an antibody, hif1an antibody, hif1an.S antibody

Background: Name/Gene ID: HIF1AN

Synonyms: HIF1AN, Factor inhibiting HIF-1, FIH1, Factor inhibiting HIF1, FIH-1

Gene ID: 55662

NCBI Accession: NP_060372

UniProt: Q9NWT6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development