Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye800CW)

Details for Product No. ABIN2169623, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6235
2031
1776
1135
782
648
372
305
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
Reactivity
Rabbit
3081
2914
2380
1662
1509
786
737
489
476
411
401
395
342
298
271
160
159
111
55
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Goat
7133
5286
1973
1077
507
362
88
84
51
35
23
15
8
5
3
1
1
Clonality
Polyclonal
Conjugate
IRDye800CW
2227
1823
1751
1266
794
718
430
343
235
147
145
140
140
116
113
112
102
98
94
93
92
91
89
84
81
80
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
10
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Supplier Product No.
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Brand In-Cell Western™,CellTag™
Immunogen Rabbit IgG
Isotype IgG
Specificity Rabbit IgG
Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
Cross-Reactivity (Details) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics The conjugate has been specifically tested and qualified for Western blotting applications.
Purification immunoaffinity chromatography
Antigen
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with m...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with se...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-M...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) ERK (green) and pERK (red) multiplex detection using infrared dye-labeled secondary a...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) p-38 (red) and Tubulin (green) multiplex detection using infrared dye-labeled seconda...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot wa...
Product cited in: Malinverni, Unterreiner, Staal, Demeyer, Galaup, Luyten, Beyaert, Bornancin: "Cleavage by MALT1 induces cytosolic release of A20." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 543-7, 2010 (PubMed).