Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye800CW)

Details for Product No. ABIN2169623
Antigen
Epitope
Heavy & Light Chain
1566
134
100
55
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Rabbit
355
341
335
232
232
121
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
8
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
841
799
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
IRDye800CW
236
219
209
149
147
130
75
49
48
35
31
26
26
26
25
25
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
15
14
13
10
9
9
9
8
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
Options
Brand In-Cell Western™,CellTag™
Immunogen Rabbit IgG
Isotype IgG
Specificity Rabbit IgG
Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
Cross-Reactivity (Details) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics The conjugate has been specifically tested and qualified for Western blotting applications.
Purification immunoaffinity chromatography
Antigen
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Supplier Images
Image no. 1 for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with m...
Image no. 2 for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-M...
Image no. 3 for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with se...
Image no. 4 for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) ERK (green) and pERK (red) multiplex detection using infrared dye-labeled secondary a...
Image no. 5 for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) p-38 (red) and Tubulin (green) multiplex detection using infrared dye-labeled seconda...
Image no. 6 for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot wa...
Product cited in: Malinverni, Unterreiner, Staal, Demeyer, Galaup, Luyten, Beyaert, Bornancin: "Cleavage by MALT1 induces cytosolic release of A20." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 543-7, 2010 (PubMed).