Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye800CW)

Details for Product No. ABIN2169623, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4781
2534
1961
1067
1022
861
306
292
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Rabbit
2897
2761
2296
1676
1453
790
626
454
395
387
359
346
298
296
221
138
135
130
41
31
29
24
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
1
Host
Goat
6785
4909
1788
923
609
392
91
68
55
18
8
5
5
5
2
1
1
Clonality
Polyclonal
Conjugate
IRDye800CW
2268
1903
1785
1360
769
686
531
294
268
227
226
201
158
141
129
110
79
75
69
55
52
48
48
45
40
39
37
35
35
29
28
28
27
27
27
27
27
27
22
19
19
19
19
17
15
15
15
15
15
15
14
12
12
11
10
10
10
9
9
9
9
8
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Supplier Product No.
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Brand In-Cell Western™,CellTag™
Immunogen Rabbit IgG
Isotype IgG
Specificity Rabbit IgG
Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
Cross-Reactivity (Details) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics The conjugate has been specifically tested and qualified for Western blotting applications.
Purification immunoaffinity chromatography
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with m...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with se...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-M...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) ERK (green) and pERK (red) multiplex detection using infrared dye-labeled secondary a...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) p-38 (red) and Tubulin (green) multiplex detection using infrared dye-labeled seconda...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot wa...
Product cited in: Malinverni, Unterreiner, Staal, Demeyer, Galaup, Luyten, Beyaert, Bornancin: "Cleavage by MALT1 induces cytosolic release of A20." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 543-7, 2010 (PubMed).