Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye800CW)

Details for Product No. ABIN2169623, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6012
1904
1868
1159
274
266
266
124
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reactivity
Rabbit
2740
2672
2191
1495
1370
700
664
447
434
362
359
354
275
258
257
147
115
84
43
26
24
21
12
12
8
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
Host
Goat
6467
4816
1755
887
458
274
87
54
42
35
22
8
6
5
4
1
1
1
Clonality
Polyclonal
Conjugate
IRDye800CW
2063
1741
1639
1144
678
644
422
315
171
122
102
102
94
89
88
88
86
84
79
78
74
73
70
68
68
67
65
65
63
55
55
54
54
54
54
54
53
53
53
53
53
48
47
47
41
40
32
31
30
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
10
10
10
10
10
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Supplier Product No.
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Brand In-Cell Western™,CellTag™
Immunogen Rabbit IgG
Isotype IgG
Specificity Rabbit IgG
Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
Cross-Reactivity (Details) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics The conjugate has been specifically tested and qualified for Western blotting applications.
Purification immunoaffinity chromatography
Antigen
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with m...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with se...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-M...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) ERK (green) and pERK (red) multiplex detection using infrared dye-labeled secondary a...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) p-38 (red) and Tubulin (green) multiplex detection using infrared dye-labeled seconda...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot wa...
Product cited in: Malinverni, Unterreiner, Staal, Demeyer, Galaup, Luyten, Beyaert, Bornancin: "Cleavage by MALT1 induces cytosolic release of A20." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 543-7, 2010 (PubMed).