Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye800CW)

Details for Product No. ABIN2169623
Antigen
Epitope
Heavy & Light Chain
840
233
200
38
13
8
Reactivity
Rabbit
222
197
165
143
124
76
75
57
53
53
44
41
40
35
23
14
13
7
7
7
3
2
Host
Goat
575
499
185
44
36
32
7
7
1
Clonality
Polyclonal
Conjugate
IRDye800CW
181
161
138
131
129
127
46
35
32
29
28
24
16
11
9
9
9
8
7
6
5
5
5
5
5
5
5
5
5
4
4
4
2
2
2
2
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
Options
Brand In-Cell Western™,CellTag™
Immunogen Rabbit IgG
Isotype IgG
Specificity Rabbit IgG
Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
Cross-Reactivity (Details) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics The conjugate has been specifically tested and qualified for Western blotting applications.
Purification immunoaffinity chromatography
Antigen
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with m...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with se...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-M...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) ERK (green) and pERK (red) multiplex detection using infrared dye-labeled secondary a...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) p-38 (red) and Tubulin (green) multiplex detection using infrared dye-labeled seconda...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) (ABIN2169623) Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot wa...
Product cited in: Malinverni, Unterreiner, Staal, Demeyer, Galaup, Luyten, Beyaert, Bornancin: "Cleavage by MALT1 induces cytosolic release of A20." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 543-7, 2010 (PubMed).