Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (IRDye680RD)

Details for Product No. ABIN2169635, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4679
2536
1881
1066
1006
860
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Rabbit
2848
2739
2296
1675
1432
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Donkey
6708
4830
1794
891
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality
Polyclonal
Conjugate
IRDye680RD
2235
1872
1744
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
48
46
43
40
38
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
10
10
9
9
9
9
9
9
8
8
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Supplier Product No.
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Brand In-Cell Western™,CellTag™
Immunogen Rabbit IgG, whole molecule
Isotype IgG
Specificity Rabbit IgG
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rabbit IgG and with the light chains common to most rabbit immunoglobulins.
Cross-Reactivity (Details) This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species.
No reactivity was detected against non-immunoglobulin serum proteins.
Purification immunoaffinity chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blot: 1:5,000 - 1:25,000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with sterile, distilled water
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light.
Storage 4 °C
Supplier Images
Immunoprecipitation (IP) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye680RD) (ABIN2169635) Palmitoylation of Lck kinase analyzed with a Click Chemistry IRDye® reagent. Lck was ...
Product cited in: Allan, Goggin, Caldwell: "Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning." in: PLoS ONE, Vol. 9, Issue 4, pp. e96200, 2014 (PubMed).