Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (IRDye680LT)

Details for Product No. ABIN2169649, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4679
2535
1881
1066
1005
860
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Goat
2848
2736
2292
1675
1432
788
613
442
380
379
358
339
291
290
208
138
135
125
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
Host
Donkey
6701
4830
1795
891
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality
Polyclonal
Conjugate
IRDye680LT
2236
1871
1740
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
48
46
43
40
38
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
10
10
9
9
9
9
9
9
8
8
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen Goat IgG
Isotype IgG
Specificity Goat IgG
Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG.
Cross-Reactivity Sheep (Ovine)
Cross-Reactivity (Details) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
Purification Immunoaffinity chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blot: 1:20,000 Recommended
Optimum dilutions will vary and should be determined empirically.

NOTE: When using PVDF membranes for Western blotting, SDS (final concentration of 0.1 – 0.2%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

  • IRDye 680LT secondary antibodies are suitable for immunofluorescent microscopy and other fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
  • IRDye 680LT is not intended for In-Cell WesternTM assays or in vivo applications.
  • These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000.
    Note: optimization may be required with IRDye 680LT. Please see pack insert for specific detergents/protocol modifications needed.
  • Comment

    • IRDye 680LT secondary antibodies have been proven to provide the brightest signal for Western blot detection in the 700 nm channel and are comparable to Alexa Fluor® 680 secondary antibodies.
    • Choose IRDye 680LT secondary antibodies to get high signal and for specific uses of detection in the 700nm channel.
    • We do not recommend to start with IRDye 680LT secondaries but rather to start with one of the IRDye 680RD secondaries.

    Restrictions For Research Use only
    Format Lyophilized
    Reconstitution Reconstitute with sterile, distilled water
    Concentration 1.0 mg/mL
    Buffer PBS, pH 7.4
    Preservative Sodium azide
    Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice Protect from light.
    Storage 4 °C
    Product cited in: Mans, Banz, Mueller, Pabst: "The angiogenesis inhibitor vasostatin is regulated by neutrophil elastase-dependent cleavage of calreticulin in AML patients." in: Blood, Vol. 120, Issue 13, pp. 2690-9, 2012 (PubMed).