Goat anti-Rabbit IgG Antibody (FITC)

Catalog No. ABIN2176511

Product Details

Target: IgG

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Purification: Purified by Protein A.

Isotype: IgG

Application Details

Application Notes: IF(IHC-P): (1:500-2000), IF(IHC-F): (1:500-2000), IF(ICC): (1:500-1000)
Optimal working dilution should be determined by the investigator.

Comment:

Exitation/Emission: 494nm/518nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4 °C for 12 months.

References

Pei, Jiao, Dong, Pei, He, Wang, Xu: "Excitotoxicity and compensatory upregulation of GAD67 in fetal rat hippocampus caused by prenatal nicotine exposure are associated with inhibition of the BDNF pathway." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 123, pp. 314-325, (2019) (PubMed).

Mao, Gao, Chen, Song, Song, Zhou, Zhao, Zhou, Wang, Zhu, Liu, Mei: "Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor." in: Cell death & disease, Vol. 8, Issue 11, pp. e3162, (2018) (PubMed).

Hong, Liu, Zhang, Wu, Hou: "Progesterone suppresses Aβ42-induced neuroinflammation by enhancing autophagy in astrocytes." in: International immunopharmacology, Vol. 54, pp. 336-343, (2018) (PubMed).

Meng, Zhang, Yuan, Zhang, Fu, Su, Zhu, Wang, Gao, Shu, Jiang, Wang: "Oleic acid stimulates HC11 mammary epithelial cells proliferation and mammary gland development in peripubertal mice through activation of CD36-Ca2+ and PI3K/Akt signaling pathway." in: Oncotarget, Vol. 9, Issue 16, pp. 12982-12994, (2018) (PubMed).

Vuletic, Zhou, Li, Bai, Meng, Zhu, Ding, Li, Sun, Ren: "Validation of Bevacizumab Therapy Effect on Colon Cancer Subtypes by Using Whole Body Imaging in Mice." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, (2017) (PubMed).

He, Lu, Dong, Jiao, Zhang, Yu, Zhang, Wang, Xu: "Prenatal nicotine exposure induces HPA axis-hypersensitivity in offspring rats via the intrauterine programming of up-regulation of hippocampal GAD67." in: Archives of toxicology, (2017) (PubMed).

Sun, Liu, Liu, Yu, Shang, Huang, Zhou, Liu, Ran, Li, Shi, Pan: "Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model." in: Scientific reports, Vol. 6, pp. 23931, (2017) (PubMed).

Liu, Liu, Hou, Cui, Li, Chi, Yi, Wen, Yin: "Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro." in: Molecular medicine reports, Vol. 14, Issue 4, pp. 3669-75, (2016) (PubMed).

Hu, Li, Qu, Li, Liu, Xia, Hongdu, Lin, Xu, Lou, He, Ma, Chen: "TMEM166/EVA1A interacts with ATG16L1 and induces autophagosome formation and cell death." in: Cell death & disease, Vol. 7, Issue 8, pp. e2323, (2016) (PubMed).

Zhang, Wang, Zheng, Zhao, Zhang, Liu, Huang: "Effects of testosterone on the expression levels of AMH, VEGF and HIF-1α in mouse granulosa cells." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 883-888, (2016) (PubMed).

Sun, Liu, Liu, Yu, Zhou, Liu, Ran, Pan, Zhang: "In vivo study of alginate hydrogel conglutinating cells to polycaprolactone vascular scaffolds fabricated by electrospinning." in: Journal of biomedical materials research. Part B, Applied biomaterials, (2016) (PubMed).

Xia, Qu, Li, Hongdu, Xu, Lin, Lou, He, Ma, Chen: "MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling." in: Autophagy, Vol. 12, Issue 9, pp. 1614-30, (2016) (PubMed).

Zhang, Wang, Zhou, Ma, Yao, Liu: "Phenotypic transformation of smooth muscle cells from porcine coronary arteries is associated with connexin 43." in: Molecular medicine reports, Vol. 14, Issue 1, pp. 41-8, (2016) (PubMed).

Liu, Dou, Zheng, Wen, Qin, Wang, Tang, Zhang, Lv, Wang, Zhao: "Curcumin upregulates Nrf2 nuclear translocation and protects rat hepatic stellate cells against oxidative stress." in: Molecular medicine reports, Vol. 13, Issue 2, pp. 1717-24, (2016) (PubMed).

Liu, Yang, Liu, Wang, Wang, Zhang, Wang, Dong, Li: "NME2 reduces proliferation, migration and invasion of gastric cancer cells to limit metastasis." in: PLoS ONE, Vol. 10, Issue 2, pp. e0115968, (2016) (PubMed).

Zhang, Zuo, Liu, Li, Tang, Xiao, Lian, Wang, Jin, Wang, Zhang, Li: "The Induction Effect of Am80 and TSA on ESC Differentiation via Regulation of Stra8 in Chicken." in: PLoS ONE, Vol. 10, Issue 11, pp. e0140262, (2016) (PubMed).

Diao, Pang, Qiu, Miao, Yu, Fan: "Construction of a human corneal stromal equivalent with non-transfected human corneal stromal cells and acellular porcine corneal stromata." in: Experimental eye research, Vol. 132, pp. 216-24, (2015) (PubMed).

Wang, Hu, Li, Qu, He, Lou, Song, Ma, Chen: "PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1." in: Autophagy, Vol. 10, Issue 12, pp. 2158-70, (2015) (PubMed).

Ding, Chen, Liu, Wu, Ding, Li, Wang: "Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos." in: Dong wu xue yan jiu = Zoological research, Vol. 36, Issue 1, pp. 54-8, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.