CCL2 antibody (Biotin)

Catalog No. ABIN2191759

Product Details anti-CCL2 Antibody (Biotin)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CCL2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Cross-Reactivity (Details): Cross reactivity: Human : No

Sterility: 0.2 μm filtered

Clone: ECE-2

Isotype: IgG1

Application Details

Application Notes: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-CCL2 antibody (Biotin) (ABIN2191759)

Tieu, Lee, Sun, Lejeune, Recinos, Ju, Spratt, Guo, Milewicz, Tilton, Brasier: "An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice." in: The Journal of clinical investigation, Vol. 119, Issue 12, pp. 3637-51, (2009) (PubMed).

Kimura, Sumiyoshi, Samukawa, Satake, Sakanaka: "Facilitating action of asiaticoside at low doses on burn wound repair and its mechanism." in: European journal of pharmacology, Vol. 584, Issue 2-3, pp. 415-23, (2008) (PubMed).

Zoja, Liu, Donadelli, Abbate, Testa, Corna, Taraboletti, Vecchi, Dong, Rollins, Bertani, Remuzzi: "Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice." in: Journal of the American Society of Nephrology : JASN, Vol. 8, Issue 5, pp. 720-9, (1997) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: Monocyte Chemoattractant Protein 1 (CCL2 Products)

Synonyms: GDCF-2 antibody, HC11 antibody, HSMCR30 antibody, MCAF antibody, MCP-1 antibody, MCP1 antibody, SCYA2 antibody, SMC-CF antibody, AI323594 antibody, JE antibody, Scya2 antibody, Sigje antibody, MCP-1A antibody, MCP1A antibody, C-C motif chemokine ligand 2 antibody, chemokine (C-C motif) ligand 2 antibody, C-C motif chemokine 2 antibody, CCL2 antibody, Ccl2 antibody, LOC101120093 antibody

Background: The monoclonal antibody ECE.2 recognizes mouse monocyte chemoattractant protein 1 (MCP-1). The murine JE gene encodes the monocyte-specific cytokine monocyte chemotactic protein 1 (MCP- 1). MCP-1 is a CC chemokine of 76 amino acids (~11 kDa) and is chemotactic for monocytes and basophils but not neutrophils and eosinophils. MCP-1 is expressed by smooth muscle cells (SMC), macrophages, endothelial cells, keratinocytes and fibroblasts in response to inflammatory stimuli such as interleukin 1β and tumor necrosis factor α. MCP-1 has been implicated in a variety of inflammatory processes, including inflammatory bowel disease, rheumatoid arthritis, asthma, nephritis, and parasitic and viral infections. MCP-1 antigen is not detected in the endothelium or SMC of normal arteries. MCP-1 has also been shown to exhibit biological activities other than chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer) MCP-1 signals via the CCR2 receptor, and is critical for aneurysm formation because of its

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection