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TLR4 antibody (Biotin)

TLR4 Reactivity: Mouse FACS, IP, Func Host: Rat Monoclonal MTS510 Biotin
Catalog No. ABIN2191769
  • Target See all TLR4 Antibodies
    TLR4 (Toll-Like Receptor 4 (TLR4))
    Reactivity
    • 179
    • 97
    • 75
    • 37
    • 23
    • 20
    • 19
    • 19
    • 5
    • 2
    • 1
    • 1
    Mouse
    Host
    • 139
    • 71
    • 4
    • 4
    Rat
    Clonality
    • 143
    • 75
    Monoclonal
    Conjugate
    • 98
    • 22
    • 21
    • 15
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TLR4 antibody is conjugated to Biotin
    Application
    • 160
    • 126
    • 61
    • 55
    • 36
    • 29
    • 27
    • 27
    • 26
    • 25
    • 12
    • 8
    • 8
    • 7
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
    Sterility
    0.2 μm filtered
    Clone
    MTS510
    Top Product
    Discover our top product TLR4 Primary Antibody
  • Application Notes
    For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive RAW264.7 cells control
    Restrictions
    For Research Use only
  • Buffer
    PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide. 1-
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
  • Burton, Gibbs, Miller, Jones, Wen, Dunne, Cooke, Zaccone: "Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens." in: European journal of immunology, Vol. 40, Issue 8, pp. 2221-9, (2010) (PubMed).

    Tsukumo, Carvalho-Filho, Carvalheira, Prada, Hirabara, Schenka, Araújo, Vassallo, Curi, Velloso, Saad: "Loss-of-function mutation in Toll-like receptor 4 prevents diet-induced obesity and insulin resistance." in: Diabetes, Vol. 56, Issue 8, pp. 1986-98, (2007) (PubMed).

    Tsujimoto, Ono, Matsumoto, Kawabata, Kinoshita, Majima, Hiraki, Seki, Moldawer, Mochizuki: "A critical role of CpG motifs in a murine peritonitis model by their binding to highly expressed toll-like receptor-9 on liver NKT cells." in: Journal of hepatology, Vol. 45, Issue 6, pp. 836-43, (2006) (PubMed).

    Cook, Trgovcich, Zimmerman, Zhang, Sedmak: "Lipopolysaccharide, tumor necrosis factor alpha, or interleukin-1beta triggers reactivation of latent cytomegalovirus in immunocompetent mice." in: Journal of virology, Vol. 80, Issue 18, pp. 9151-8, (2006) (PubMed).

    Ortega-Cava, Ishihara, Rumi, Kawashima, Ishimura, Kazumori, Udagawa, Kadowaki, Kinoshita: "Strategic compartmentalization of Toll-like receptor 4 in the mouse gut." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 8, pp. 3977-85, (2003) (PubMed).

    Nomura, Akashi, Sakao, Sato, Kawai, Matsumoto, Nakanishi, Kimoto, Miyake, Takeda, Akira: "Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3476-9, (2000) (PubMed).

    Sato, Nomura, Kawai, Takeuchi, Mühlradt, Takeda, Akira: "Synergy and cross-tolerance between toll-like receptor (TLR) 2- and TLR4-mediated signaling pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7096-101, (2000) (PubMed).

  • Target
    TLR4 (Toll-Like Receptor 4 (TLR4))
    Abstract
    TLR4 Products
    Synonyms
    ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody
    Background
    The monoclonal antibody MTS510 reacts with the Toll-like receptor 4 (TLR4, CD284) that is associated with MD2. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single α-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4, CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways, one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of pro-inflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR-domaincontaining adaptor protein inducing interferon-β) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomer and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells. The monoclonal antibody MTS510 reacts preferentially, especially in flow cytometry, with mouse TLR4 that is associated with MD-2. MTS510 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to coprecipitate MD-2 (30 kDa) with TLR4 (100 kDa). Aliases Toll-like receptor 4: TLR4, CD284, ARMD10 MD2: ESOP-1, myeloid differentiation protein-2, lymphocyte antigen 96 (Ly-96) 2 Immunogen Ba/F3 cells expressing mouse TLR4 and MD-2
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
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