ARHGEF2 antibody

Catalog No. ABIN2192013

Product Details anti-ARHGEF2 Antibody

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARHGEF2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Cross-Reactivity (Details): Cross reactivity: Canine : Yes, Mouse (=Lfc) : Yes

Sterility: 0.2 μm filtered

Clone: B4-7

Isotype: IgG1

Application Details

Application Notes: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. 1

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-ARHGEF2 antibody (ABIN2192013)

Tonami, Kurihara, Arima, Nishiyama, Uchijima, Asano, Sorimachi, Kurihara: "Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1." in: Journal of cell science, Vol. 124, Issue Pt 8, pp. 1214-23, (2011) (PubMed).

Saito, Murata-Kamiya, Hirayama, Ohba, Hatakeyama: "Conversion of Helicobacter pylori CagA from senescence inducer to oncogenic driver through polarity-dependent regulation of p21." in: The Journal of experimental medicine, Vol. 207, Issue 10, pp. 2157-74, (2010) (PubMed).

Aijaz, DAtri, Citi, Balda, Matter: "Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition." in: Developmental cell, Vol. 8, Issue 5, pp. 777-86, (2005) (PubMed).

Benais-Pont, Punn, Flores-Maldonado, Eckert, Raposo, Fleming, Cereijido, Balda, Matter: "Identification of a tight junction-associated guanine nucleotide exchange factor that activates Rho and regulates paracellular permeability." in: The Journal of cell biology, Vol. 160, Issue 5, pp. 729-40, (2003) (PubMed).

Target Details for ARHGEF2

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Alternative Name: Guanine Nucleotide Exchange Factor h1 (ARHGEF2 Products)

Synonyms: MGC82333 antibody, MGC86214 antibody, MGC75855 antibody, AA408978 antibody, GEF antibody, GEF-H1 antibody, GEFH1 antibody, LFP40 antibody, Lbcl1 antibody, Lfc antibody, P40 antibody, mKIAA0651 antibody, GEF-H1/LFC antibody, Rho/Rac guanine nucleotide exchange factor 2 antibody, Rho/Rac guanine nucleotide exchange factor 2 S homeolog antibody, rho/rac guanine nucleotide exchange factor (GEF) 2 antibody, ARHGEF2 antibody, arhgef2.S antibody, arhgef2 antibody, Arhgef2 antibody

Background: The monoclonal antibody B4/7 recognizes human and canine guanine nucleotide exchange factor H1 (GEF-H1). GEFH1 is an ~110 kDa protein belonging to the Dbl family of proto-oncogenes. GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of epithelial tight junctions and the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin. GEF-H1 is part of the signaling pathway connecting epithelial polarity with the cell cycle, and as such involved in oncogenesis. Aliases Rho guanine nucleotide exchange factor 2 (ARHGEF2), Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40, KIAA0651, LFP40 1 Immunogen protein fraction isolated from detergent extracts of MDCK cells .

Pathways: Negative Regulation of intrinsic apoptotic Signaling