CD46 antibody

Catalog No. ABIN2192126

Product Details anti-CD46 Antibody

Target: CD46

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD46 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: MM-1

Application Details

Application Notes: For immunohistology, immunofluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Western blotting: rat testis lysate, non-reducing conditions, band size 38 kDa (Ref. 1and 4) control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-CD46 antibody (ABIN2192126)

Mizuno, Harris, Morgan: "Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury." in: Journal of reproductive immunology, Vol. 69, Issue 1, pp. 23-34, (2006) (PubMed).

Mizuno, Donev, Harris, Morgan: "CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa." in: Molecular immunology, Vol. 44, Issue 7, pp. 1613-22, (2006) (PubMed).

Mizuno, Harris, Suzuki, Matsuo, Morgan: "Expression of CD46 in developing rat spermatozoa: ultrastructural localization and utility as a marker of the various stages of the seminiferous tubuli." in: Biology of reproduction, Vol. 72, Issue 4, pp. 908-15, (2005) (PubMed).

Mizuno, Harris, Johnson, Morgan: "Rat membrane cofactor protein (MCP; CD46) is expressed only in the acrosome of developing and mature spermatozoa and mediates binding to immobilized activated C3." in: Biology of reproduction, Vol. 71, Issue 4, pp. 1374-83, (2004) (PubMed).

Target Details for CD46

Target: CD46

Alternative Name: CD46 (CD46 Products)

Synonyms: AHUS2 antibody, MCP antibody, MIC10 antibody, TLX antibody, TRA2.10 antibody, Mcp antibody, CD46 antibody, CD46 molecule antibody, CD46 antigen, complement regulatory protein antibody, CD46 molecule, complement regulatory protein antibody, membrane cofactor protein-like antibody, CD46 antibody, Cd46 antibody, LOC100341359 antibody, LOC103789310 antibody

Background: The monoclonal antibody MM.1 recognizes CD46, also known as membrane cofactor protein. An immunohistological survey of rat tissues revealed that CD46 is expressed only in the testis and epididymis of adult rats more specifically, only by spermatozoa and late spermatozoal precursors. In contrast, in humans, CD46 is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to down-regulate the activation of complement on host tissue. It functions as a cofactor by binding to C3b and C4b. Binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. In human, it also serves as a receptor for measles virus. Two isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. The isoforms differ in the length. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). Aliases Membrane cofactor protein Immunogen Second and third SCR domains of MCP fused to IgG-Fc, MCP23-Ig

Pathways: Regulation of Actin Filament Polymerization