Muscarinic Acetylcholine Receptor M2 antibody

Catalog No. ABIN2192224

Product Details anti-Muscarinic Acetylcholine Receptor M2 Antibody

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Muscarinic Acetylcholine Receptor M2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Functional Studies (Func), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: B8E5

Application Details

Application Notes: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

References for anti-Muscarinic Acetylcholine Receptor M2 antibody (ABIN2192224)

Nascimento, Sallé, Hoebeke, Argibay, Peineau: "cGMP-mediated inhibition of cardiac L-type Ca(2+) current by a monoclonal antibody against the M(2) ACh receptor." in: American journal of physiology. Cell physiology, Vol. 281, Issue 4, pp. C1251-8, (2001) (PubMed).

Elies, Fu, Eftekhari, Wallukat, Schulze, Granier, Hjalmarson, Hoebeke: "Immunochemical and functional characterization of an agonist-like monoclonal antibody against the M2 acetylcholine receptor." in: European journal of biochemistry / FEBS, Vol. 251, Issue 3, pp. 659-66, (1998) (PubMed).

Target Details for Muscarinic Acetylcholine Receptor M2

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Alternative Name: Muscarinic Acetylcholine Receptor m2 (CHRM2 Products)

Synonyms: HM2 antibody, AChR-M2 antibody, Chrm-2 antibody, M2 antibody, chrm2 antibody, Acm2 antibody, GPM2 antibody, cholinergic receptor muscarinic 2 antibody, cholinergic receptor, muscarinic 2, cardiac antibody, cholinergic receptor, muscarinic 2a antibody, cholinergic receptor, muscarinic 2 antibody, CHRM2 antibody, Chrm2 antibody, chrm2a antibody

Background: The monoclonal antibody B8E5 recognizes human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa. This receptor is an integral membrane protein consisting of seven membrane spanning α-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. 2+ Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP. Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor. M2ACh-R Aliases Peptide VRTVEDGECYIQFFSNAAVTFGTAI(C): Human second extracellular loop residues 168-192 Immunogen Mouse IgG2a