p53 antibody (Tumor Protein P53) (Ser20)

Details for Product anti-TP53 Antibody No. ABIN222890, Supplier: Log in to see
Antigen
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • transformation related protein 53, pseudogene
  • Wistar clone pR53P1 p53 pseudogene
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
  • Trp53-ps
  • p53-ps
Alternatives
anti-Human p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Ser20
173
128
89
83
73
71
70
61
58
54
49
48
47
47
41
38
32
30
28
23
22
20
17
16
15
15
15
15
15
15
14
12
11
11
10
10
9
9
9
8
8
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2376
513
426
190
127
94
87
84
35
23
20
20
19
16
15
14
6
4
4
4
3
3
3
2
1
1
Host
Mouse
1349
1138
7
3
1
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This p53 antibody is un-conjugated
81
78
65
54
47
46
45
31
31
26
25
25
25
25
25
24
24
24
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1902
770
743
735
626
623
434
344
317
179
72
41
32
31
21
17
17
15
13
9
9
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Synthetic peptide corresponding to residue surrounding Ser20 of human p53
Blocking Peptide Blocking peptide for this product available: ABIN412862
Clone C6A5
Isotype IgG1
Purification Affinity purified
Alternative Name p53 (TP53 Antibody Abstract)
Background P53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Synonyms: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
Application Notes Western blotting (0.5-4 μg/mL), immunoprecipitation (4-8 μg/mL), Immunohistochemistry (4-8 μg/mL), immunocytochemistry (4-8 μg/mL). However, the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) in PBS containing 1 mg/mL BSA and 1.5 mM sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (Ser20) antibody (ABIN222890) Western blot analysis of p53 in Cos cell lysate.
Product cited in: Javed, Khan, Khan, Vaibhav, Ahmad, Khuwaja, Ahmed, Raza, Ashafaq, Tabassum, Siddiqui, El-Agnaf, Safhi, Islam: "S-allyl cysteine attenuates oxidative stress associated cognitive impairment and neurodegeneration in mouse model of streptozotocin-induced experimental dementia of Alzheimer's type." in: Brain research, Vol. 1389, pp. 133-42, 2011 (PubMed).

Khan, Ishrat, Ahmad, Hoda, Khan, Khuwaja, Srivastava, Raza, Islam, Ahmad: "Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 255-63, 2009 (PubMed).

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