BID antibody (BH3 Interacting Domain Death Agonist)

Details for Product anti-BID Antibody No. ABIN222962
Antigen
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist S homeolog
  • BID
  • Bid
  • bida
  • bid.S
  • bid
Alternatives
anti-Human BID antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human
318
160
80
5
3
3
2
Host
Rabbit
311
76
21
6
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
26
21
13
8
7
7
6
6
6
6
6
6
3
3
3
3
3
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
329
166
151
77
46
41
29
20
15
11
10
9
3
2
2
1
1
1
1
1
Options
Immunogen Synthetic peptide surrounding amino acid 37 of human Bid
Blocking Peptide Blocking peptide for this product available: ABIN412616
Isotype IgG
Specificity The antibody detects 22 kDa human Bid .
Purification Affinity purified
Plasmids, Primers & others Plasmids, Primers & others BID products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
Gene ID 637
UniProt P55957
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Application Notes Western blot analysis (0.5-4 μg/mL), immunoprecipitation (5-10 μg/mL), and Immunohistochemistry (20-40 μg/mL). However, the optimal conditions should be determined individually. The antibody detects 22 kDa human Bid
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN222962) Western blot analysis of Bid expression in Jurkat cell lysate.
Product cited in: Liang, Yang, Huang, Lin, Chou, Chen, Pan: "Antegrade retrieval of ureteral stents through an 8-French percutaneous nephrostomy route." in: AJR. American journal of roentgenology, Vol. 191, Issue 5, pp. 1530-5, 2008 (PubMed).

Chen, Huang, Wang: "Deficiency of Bim in dendritic cells contributes to overactivation of lymphocytes and autoimmunity." in: Blood, Vol. 109, Issue 10, pp. 4360-7, 2007 (PubMed).

Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007 (PubMed).

Ju, Valencia, Pang, Ke, Gao, Dong, Liu: "Proteome-wide identification of family member-specific natural substrate repertoire of caspases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14294-9, 2007 (PubMed).

Kulik, Carson, Vomastek, Overman, Gooch, Srinivasula, Alnemri, Nunez, Weber: "Tumor necrosis factor alpha induces BID cleavage and bypasses antiapoptotic signals in prostate cancer LNCaP cells." in: Cancer research, Vol. 61, Issue 6, pp. 2713-9, 2001 (PubMed).

Wang, Wieckowski, Goldstein, Gastman, Rabinovitz, Gambotto, Li, Fang, Yin, Rabinowich: "Resistance to granzyme B-mediated cytochrome c release in Bak-deficient cells." in: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1325-37, 2001 (PubMed).

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