Caspase 12 (CASP12) antibody

Details for Product No. ABIN223032, Supplier: Log in to see
Antigen
  • Caspase-12
  • caspase 12
  • Casp12
  • CASP12
Alternatives
anti-Human Caspase 12 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Mouse (Murine), Rat (Rattus)
101
99
69
Host
Rabbit
128
14
12
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
140
62
60
39
18
16
15
13
10
6
3
1
Options
Supplier
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Immunogen Synthetic peptide corresponding to residues surrounding amino acid 310 of mouse caspase-12
Blocking Peptide Blocking peptide for this product available: ABIN412648
Isotype IgG
Specificity It detects procaspase-12 (55 kDa) and cleavage caspase-12 (36 kDa) on SDS-PAGE immunoblots.
Purification Affinity purified
Antigen
Alternative Name Caspase -12 (CASP12 Antibody Abstract)
Background Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains two pathways, in which the key apoptotic proteases caspase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is located to the ER, but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium hemeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and β-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-β. Caspase-12 is ubiquitously expressed in mouse tissues.
Synonyms: CASP12 , CASP12P1
Gene ID 12364
NCBI Accession NM_009808
Pathways ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response
Application Notes Western blotting (0.5-4 μg/mL). It detects procaspase-12 (55 kDa) and cleavage caspase-12 (36 kDa) on SDS-PAGE immunoblots. However, the optimal conditions should be determined individually.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-caspase-12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 12 (CASP12) antibody (ABIN223032) Western blot analysis of Caspase-12 with rat kidney tissue lysate.
Product cited in: Wang, Huang, Ochs, Li, Jin, Tang, Du: "Effect of sulfur dioxide preconditioning on rat myocardial ischemia/reperfusion injury by inducing endoplasmic reticulum stress." in: Basic research in cardiology, 2011 (PubMed).

Da?nes, Lakk, Czotter, Ga?briel: "A Precise Temporal Dissection of Monosodium Glutamate-Induced Apoptotic Events in Newborn Rat Retina In Vivo." in: Neurochemical research, 2011 (PubMed).

French, Hamilton, Quindry, Lee, Upchurch, Powers: "Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 2862-71, 2008 (PubMed).

Liang, Yang, Huang, Lin, Chou, Chen, Pan: "Antegrade retrieval of ureteral stents through an 8-French percutaneous nephrostomy route." in: AJR. American journal of roentgenology, Vol. 191, Issue 5, pp. 1530-5, 2008 (PubMed).

Chin, Lin, Kuo, Chueh: "Dual effect of thapsigargin on cell death in porcine aortic smooth muscle cells." in: American journal of physiology. Cell physiology, Vol. 292, Issue 1, pp. C383-95, 2007 (PubMed).

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